Making Matsoni Yogurt with an Instant Pot Yogurt Recipe and Temperatures for Homemade Yogurt

We made Matsoni yogurt from milk and a starter culture. This post includes an Instant Pot Yogurt Recipe and temperatures for making all kinds of Homemade yogurt.
The ingredients in this recipe reflect a commitment to sustainability and thoughtful sourcing. The milk comes from organic dairies focused on long-term land stewardship, using sustainable agricultural practices. The culture is a real Matsoni yogurt culture. The blackberries are from a local grower selling at the farmers’ market. Even the blueberry powder supports growers by providing a second use for fruit harvested in abundance.
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Estimated reading time: 6 minutes
Table of contents
What is Matsoni Yogurt
Matsoni yogurt is cultured milk product from the Armenia. It is an heirloom yogurt, which means it can be made again, indefinitely, by adding some of the previous batch to milk to make the next batch. It is also mesophilic, which means it is cultured at a lower temperature. The mild, honey-like flavor makes this perfect as a dessert.
Ingredients
By selecting better brands and eating more healthy foods we cut our carbon footprint in half and are helping to control climate change.
Milk
We use Horizon organic milk because of the taste and quality, and because they are working hard at becoming carbon positive to help the environment.
Culture
The yogurt starter culture is from Cultures for Health. Since Matsoni yogurt is an heirloom variety, it is possible to make successive batches of yogurt from a few tablespoons of a previous batch, but we usually start with fresh starter culture to make sure the culture is strong and the milk is kept clean. The starter culture comes in powdered form in tiny packets and conveniently stores for months in the refrigerator. It also comes with instructions, but the method we describe here is just easier.
Fruit
We sweetened it and served it with blackberry juice, blackberries and blueberry powder.
Method
This recipe uses an easy, foolproof method that requires only a few minutes of active time. Check out Cultured Dairy at Home for a more detailed explanation on how this works.
- Bring the milk to a temperature that will kill bacteria and cause the protein to thicken the product
- Allow to cool to the correct temperature
- Mix in the Matsoni yogurt culture
- Keep at the correct temperature required for the particular starter, and for the required amount of time
- Chill
This recipe uses our method for making Cultured Dairy at Home. An easy, foolproof method.
Sustainability
Every ingredient we choose has an impact. The good news is that small, thoughtful decisions in the kitchen can support more sustainable farming practices and better food systems overall.
My experience working in agriculture taught me how much these choices matter—from animal health to water use to long-term sustainability. This recipe reflects those values. In the Ingredient section above, you’ll find simple insights into the choice of ingredients, so you can make informed choices without overcomplicating the process.
Mesophilic Culture
There are a variety of different yogurt cultures. They can be grouped into two categories – mesophilic and thermophilic. It is important to know which category your yogurt starter is in, because they thrive at different temperatures. Matsoni Yogurt is made from a mesophilic culture.
- Mesophilic, which translates to “medium-loving”, indicates that it thrives at medium temperatures (around 68-100 degrees F) and is destroyed at a higher temperature (around 113 degrees F).
- Thermophilic, which translates to “heat-loving”, indicates that it thrives at higher temperatures (around 105-115 degrees F) and is destroyed at a higher temperature (around 130 degrees F). Most yogurt cultures are thermophilic.
Making Matsoni Yogurt in an Instant Pot
We wanted to make real yogurt, so that we would know exactly what was in it. The yogurt would be just high quality milk with a yogurt starter culture – no sugar or additives. But making yogurt at home can be a little tricky, because you need control the temperature for a number of hours, and not all yogurts are cultured at the same temperature. Also, you may want to boil the milk before culturing, which requires another temperature.
Making yogurt in an Instant Pot is perfect for this. It has a special yogurt setting that scalds the milk, and then another setting that allows you to select the temperature and it will hold it there for hours. The Instant Pot Ultra has a yogurt setting that can be adjusted to low, where it will maintain a constant temperature of 91 degrees, which will usually work for culturing a mesophilic culture. The medium setting will culture a thermophilic culture.
Also, making it in an Instant Pot is easier, and a great way to use less energy. We use an Instant Pot Ultra, that has a special yogurt setting. This creates incredible yogurt, allowing us to eat less meat, and also saves money.

Because this is a cultured product, it is important that everything is clean. Start with milk that is not near its expiration date. Remove the sealing ring on the Instant Pot lid. Thoroughly wash the sealing ring, the inner pot and the lid with very hot water and put the sealing ring back on.
For this yogurt, boiling the milk is not required, but will produce a thicker yogurt. If you decide to boil the milk, use the boil setting on the yogurt setting to bring the temperature of the milk above 180 degrees F and then let it cool to below 91 degrees F before adding the Matsoni yogurt starter culture. This will kill any unwanted bacteria, and also help produce a thicker yogurt.
The milk and starter culture must be kept at a constant temperature where the culture will thrive. Matsoni yogurt is a mesophilic variety, so it needs to be kept between 68 and 100 degrees F. The low setting on the Instant Pot is 91 degrees F, so this setting will work. The longer it is cultured the thicker and more tart it becomes.
That’s it – no sugar, no additives.

RECIPE
Other Recipes Made with Cultured Dairy
These recipes use our method for making Cultured Dairy at Home. An easy, foolproof method.
True to The Master Pantry philosophy, this recipe combines the best seasonal ingredients and quality brands with homemade methods to maximize both flavor and sustainability. It was inspired by our travels and works well as a holiday recipe.
Photos by Tony Fitzgerald Photography
Recipes created by Lisa LeCoump — Food Photographer, Agricultural Expert, and Home Baker. Sharing master recipes, chef secrets, and sustainable baking for every kitchen.









